Identifying proteins that bind a known RNA sequence using the yeast three-hybrid system.
نویسندگان
چکیده
The yeast three-hybrid system can be used to identify a protein partner of a known RNA sequence by screening a cDNA library fused to a transcription activation domain, with a hybrid RNA as 'bait.' Most commonly, such screens are performed to identify proteins that interact with a given RNA in vivo.
منابع مشابه
Identification of RNAs that bind to a specific protein using the yeast three-hybrid system.
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ورودعنوان ژورنال:
- Methods in enzymology
دوره 539 شماره
صفحات -
تاریخ انتشار 2014